Core F: Multi-Omics Core
The Multi-Omics Core seeks to develop and apply technologies based on mass spectrometry (MS), coupled with transcriptome studies, to uncover proteomic, metabolomic, and lipidomic changes. Omics assessments will be made directly using human tissues and biofluids, human organoids, mouse and cell models, as well as isolated cellular compartments and protein complexes linked to AD in an apoE isoform-dependent manner. To achieve this goal, we have assembled a strong multidisciplinary team with established and renowned investigators in proteomics (Peng), metabolomics (Peng) and lipidomics (Han), both having extensive expertise in studying neurodegeneration. The Peng lab has been developing high-throughput proteomics approaches and metabolomics methods, enabling the analysis of >10,000 proteins, diverse post-translational modification sites, and >1,000 metabolites. The Han lab has developed innovative lipidomics technologies for comprehensive, accurate, and effective analysis of cellular lipidome. The latest MS platform allows the profiling of nearly complete brain lipidome (n = ~1,500 species). Core F will apply the latest proteomics, metabolomics and lipidomics strategies across U19 components.