Neural Induction for Neural Progenitors, Neurons, and Astrocytes
Preparation of Tissue Culture Plates for Neural Rosettes and Neural Progenitors
Neural Aggregate Formation
Neural Rosette Formation and Selection
Neural Progenitor Expansion
Neural Progenitor Banking
Neural Rosette Banking
Thawing Rosettes and NPC
Differentiation of NPC into Cortical Neurons
iPSC Cortical Differentiation
Seamless Genome Editing of iPSC
Genomic Editing of iPSC
Footprint-Free Genome Editing of iPSC Using Alt-R CRISPR/Cas9
Nucleofection of iPSC
Serial Dilution of Nucleofected iPSC Pools
Picking Clones
Splitting 96 Well Plates for gDNA Extraction and Continuing Culture
Splitting 96 Well Plates for gDNA Extraction and Freezing Down
Screening Edited iPSC Clones
iPSC gDNA Extraction for Screening Edited Clones
iPSC PCR for Screening Edited Clones
iPSC Restriction Digest for Screening Edited Clones
Sanger Sequencing
Thawing iPSC Plate
Microglia
Differentiation of iPSC into Microglia-Like Cells (iMGL)
Fibroblasts
Thawing, Expanding, and Freezing Human Fibroblasts
Astrocytes
An Efficient Platform for Astrocyte Differentiation from Human Induced Pluripotent Stem Cells
Cerebral Organoids
For information on Cerebral Organoid culture, please refer to ELAVL4, splicing and glutamatergic dysfunction precede neuron loss in MAPT mutation cerebral organoids
